Spectrofluorimetric Method of Analysis for Gabapentin in Spiked Human Plasma and Formulations

Gabapentin is an anticonvulsant widely used in the treatment of epilepsy.A simple, rapid, accurate, precise and economic spectrofluorimetric methods have been developed for the estimation of gabapentin in pharmaceutical formulations and in spiked human plasma. The analysis and validation dine by using SHIMADZU RF – 5301 Spectofluorimeter, equipped with Xenon arc lamp, using quartz cell (1×1×4.5) cm, with R-450-01photomultiplier detector. The absorbance was found to increase linearly with increasing concentration of gabapentin over the concentration range of 0.2 – 2.0 μg/ml for Gabapentin with a mean r value 0.9989. Recovery studies gave satisfactory results indicating that none of common additives and excipients interfere the assay method. Various analytical parameters were evaluated and the results were validated by statistical data. The proposed method was found to be simple, sensitive, accurate, precise and economic and can be successfully applied for the analysis and validation of gabapentin in both pharmaceutical dosage form and Human plasma.